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The human genome is too long to be sequenced as one continuous string by current technology – so short-read sequencing breaks DNA into short fragments that are amplified and then sequenced to produce ‘reads’ of around 150 nucleotides in length. To address these issues, scientists and clinicians are starting to look at ‘long-read’ sequencing.
This can be done through various techniques such as PCR, DNA sequencing or gene synthesis. Vector Construction: Vector construction involves designing and assembling a DNA molecule (vector) that carries the gene encoding the protein of interest along with regulatory elements necessary for protein expression.
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