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However, Dr. Bahassi reported that the selection of CTCs using this method is often not very efficient and the CTC population is usually highly contaminated with leukocytes. Optimal methods for bioinformatic analysis are still needed. In addition, some CTCs express the CD45 marker, and such CTCs would be eliminated with this method.
These methods often require extensive sample preparation, complex bioinformatics or specialized lab environments, making them less ideal for quality control and release testing, where multiple mRNA constructs are already mixed.
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